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Validation Data Gallery

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  • Western Blot (WB) analysis of Mo7e cells using CD117/c-Kit Recombinant antibody (84259-5-RR)

    WB analysis of Mo7e using 84259-5-RR (same clone as 84259-5-PBS)

    Mo7e cells were subjected to SDS PAGE followed by western blot with 84259-5-RR (CD117/c-Kit antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84259-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD117/c-Kit Recombinant antibody (84259-5-RR)

    IHC staining of human colon using 84259-5-RR (same clone as 84259-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-5-RR (CD117/c-Kit antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84259-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Mo7e cells using CD117/c-Kit Recombinant antibody (84259-5-RR)

    IF Staining of Mo7e using 84259-5-RR (same clone as 84259-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Mo7e cells using CD117/c-Kit antibody (84259-5-RR, Clone: 241313C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84259-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84259-5-RR

    Affinity and Kinetic Characterization of 84259-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84259-5-RR against Human CD117/c-Kit were performed. The affinity constant is 0.90 nM.

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CD117/c-Kit Recombinant antibody, PBS Only

CD117/c-Kit Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84259-5-PBS
Clone No.241313C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84259-5-PBS targets CD117/c-Kit in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Calculated molecular weight 976 aa, 110 kDa
Observed molecular weight140 kDa
GenBank accession numberBC071593
Gene Symbol CD117
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10721
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).