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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD117/c-Kit Polyclonal antibody (31337-1-AP)

    WB analysis using 31337-1-AP

    MC/9 cells were subjected to SDS PAGE followed by western blot with 31337-1-AP (CD117/c-Kit antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD117/c-Kit Polyclonal antibody (31337-1-AP)

    WB analysis using 31337-1-AP

    Mouse testis tissue was subjected to SDS PAGE followed by western blot with 31337-1-AP (CD117/c-kit antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CD117/c-Kit Polyclonal antibody

CD117/c-Kit Polyclonal Antibody for WB, ELISA
Cat No. 31337-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Kit, CD117, c KIT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMC/9 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31337-1-AP targets CD117/c-Kit in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name kit oncogene
Calculated molecular weight109 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_001122733.1
Gene Symbol CD117
Gene ID (NCBI) 16590
RRIDAB_3669950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05532-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocols

Product Specific Protocols
WB protocol for CD117/c-Kit antibody 31337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols