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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD116 (4H1)

CD116 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

4H1

Cat no : 65160-1-Ig

Synonyms

CD116, CDw116, CSF2R, CSF2RA, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM CSF R alpha, GMCSFR, GMCSFR alpha, GMR, GMR alpha

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood monocytes using 65160-1-Ig

    FC experiment of human peripheral blood monocytes using 65160-1-Ig

    1X10^6 human peripheral blood monocytes were surface stained with 0.5 ug Anti-Human CD116 (65160-1-Ig, Clone: 4H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood monocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65160-1-Ig targets CD116 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen CD116-transfected COS cells 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Calculated molecular weight 46 kDa
GenBank accession numberBC002635
Gene symbol CSF2RA
Gene ID (NCBI) 1438
RRIDAB_2918442
Conjugate Unconjugated
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer TBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CD116 antibody 65160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols