Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00094 targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
FusionProtein 相同性解析による交差性が予測される生物種 |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 100 kDa~ 120 kDa |
| GenBank accession number | NM_002294.3 |
| Gene Symbol | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | P13473-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107b / LAMP2 antibody RMX00094 | Download protocol |
| IHC protocol for CD107b / LAMP2 antibody RMX00094 | Download protocol |
| WB protocol for CD107b / LAMP2 antibody RMX00094 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |