Validation Data Gallery

Filter: AllWBIHC

WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis of pig liver using 66301-1-Ig (same clone as 66301-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CD107b / LAMP2 Monoclonal antibody, PBS Only

CD107b / LAMP2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66301-1-PBS

Clone No.2D3B9

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Synonyms

CD107b, LAMP2, 2D3B9, CD107 antigen-like family member B, LAMP 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66301-1-PBS targets CD107b / LAMP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity	human, mouse, pig
Host / Isotype	Mouse / IgA
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0595 Product name: Recombinant human LAMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-390 aa of BC002965 Sequence: NPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTILIPIIVGAGLSGLII 相同性解析による交差性が予測される生物種
Full Name	lysosomal-associated membrane protein 2
Calculated molecular weight	45 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC002965
Gene Symbol	LAMP2
Gene ID (NCBI)	3920
ENSEMBL Gene ID	ENSG00000005893
RRID	AB_2881684
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Thiophilic affinity chromatograph
UNIPROT ID	P13473
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

WB analysis of pig liver using 66301-1-Ig (same clone as 66301-1-PBS)

WB analysis of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)