Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
RMX00025 targets CD107a / LAMP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | lysosomal-associated membrane protein 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 40-48 kDa, 90-120 kDa |
| GenBank accession number | NM_005561.4 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | N/A |
| UNIPROT ID | P11279-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}} |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107a / LAMP1 antibody RMX00025 | Download protocol |
| IP protocol for CD107a / LAMP1 antibody RMX00025 | Download protocol |
| WB protocol for CD107a / LAMP1 antibody RMX00025 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |