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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis of HeLa using 67300-1-Ig (same clone as 67300-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis of HepG2 using 67300-1-Ig (same clone as 67300-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis of Jurkat using 67300-1-Ig (same clone as 67300-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis of THP-1 using 67300-1-Ig (same clone as 67300-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CD107a / LAMP1 Monoclonal antibody (67300-1-Ig)

    IF Staining of human liver using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD107a / LAMP1 antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

CD107a / LAMP1 Monoclonal antibody, PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67300-1-PBS
Clone No.3B2E9

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67300-1-PBS targets CD107a / LAMP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28869

Product name: Recombinant human CD107a protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 35-250 aa of BC021288

Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNT

相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight45 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2882564
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11279
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

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