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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD107a / LAMP1 Monoclonal antibody, PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B2E9

Cat no : 67300-1-PBS

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

Validation Data Gallery

Applications:
  • WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis of Jurkat using 67300-1-Ig (same clone as 67300-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis of THP-1 using 67300-1-Ig (same clone as 67300-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis of HeLa using 67300-1-Ig (same clone as 67300-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis of HepG2 using 67300-1-Ig (same clone as 67300-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67300-1-PBS targets CD107a / LAMP1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD107a / LAMP1 fusion protein Ag28869 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC021288
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.