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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD107a / LAMP1 Polyclonal antibody (32435-1-AP)

    WB analysis using 32435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD107a / LAMP1 Polyclonal antibody (32435-1-AP)

    WB analysis using 32435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CD107a / LAMP1 Polyclonal antibody (32435-1-AP)

    IF Staining of NIH/3T3 using 32435-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Lamp1 antibody (32435-1-AP) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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CD107a / LAMP1 Polyclonal antibody

CD107a / LAMP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32435-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CD107a, Lamp1, Lamp 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, C6 cells, HSC-T6 cells, rat kidney tissue, RAW 264.7 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32435-1-AP targets CD107a / LAMP1 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD107a / LAMP1 fusion protein Ag34623 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 44 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberNM_001317353
Gene Symbol CD107a
Gene ID (NCBI) 16783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP11438
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 32435-1-APDownload protocol
IF protocol for CD107a / LAMP1 antibody 32435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols