CD107a / LAMP1 Polyclonal antibody

CD107a / LAMP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21997-1-AP

Synonyms

CD107a, LAMP1, LAMPA, LAMP-1, LAMP 1


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inHepG2 cells, U937 cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21997-1-AP targets CD107a / LAMP1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig, monkey, zebrafish, bovine, turtle
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD107a / LAMP1 fusion protein Ag17367 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC021288
Gene symbol LAMP1
Gene ID (NCBI) 3916
RRIDAB_2878966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
IHC protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
IF protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Immunity

The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.

Authors - Meihong Deng
human

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou
WB

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura
human

Mol Ther

Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase

Authors - Yang Wang
human

Nat Commun

Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.

Authors - Nindo B Punturi
humanWB

J Adv Res

Dehydrocostus lactone suppresses gastric cancer progression by targeting ACLY to inhibit fatty acid synthesis and autophagic flux

Authors - Yuxuan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zakia (Verified Customer) (05-22-2024)

Works great.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: N2A,293T cells, IMG
FH

Marina (Verified Customer) (08-08-2022)

Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human breast cancer cells
CD107a / LAMP1 Antibody Western Blot validation (1:1000 dilution) in Human breast cancer cells (Cat no:21997-1-AP)
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells
FH

Mayru (Verified Customer) (04-30-2019)

The antibody works great for detection of LAMP1 protein expression using Western blot analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H4 cells