CD107a / LAMP1 Polyclonal antibody

CD107a / LAMP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 21997-1-AP

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker



Tested Applications

Positive WB detected inHepG2 cells, U937 cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human gliomas tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

21997-1-AP targets CD107a / LAMP1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity turtle, human, monkey, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD107a / LAMP1 fusion protein Ag17367 相同性解析による交差性が予測される生物種
Full Name lysosomal-associated membrane protein 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 90-120 kDa
GenBank accession numberBC021288
Gene symbol LAMP1
Gene ID (NCBI) 3916
RRIDAB_2878966
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
IHC protocol for CD107a / LAMP1 antibody 21997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Immunity

The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.

Authors - Meihong Deng
human

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou
WB

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura
human

Mol Ther

Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase

Authors - Yang Wang
human

Nat Commun

Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.

Authors - Nindo B Punturi
humanWB

Sci Total Environ

TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles

Authors - Xingyao Pei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marina (Verified Customer) (08-08-2022)

Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human breast cancer cells
CD107a / LAMP1 Antibody Western Blot validation (1:1000 dilution) in Human breast cancer cells (Cat no:21997-1-AP)
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells
FH

Mayru (Verified Customer) (04-30-2019)

The antibody works great for detection of LAMP1 protein expression using Western blot analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H4 cells