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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD100 Polyclonal antibody (27516-1-AP)

    WB analysis using 27516-1-AP (same clone as 27516-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27516-1-AP (CD100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD100 Polyclonal antibody (27516-1-AP)

    WB analysis of HeLa using 27516-1-AP (same clone as 27516-1-PBS)

    WB result of CD100 antibody (27516-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD100 Polyclonal antibody (27516-1-AP)

    IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CD100 Polyclonal antibody, PBS Only

CD100 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27516-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

SEMA4D, A8, CD 100, CD100 antigen, SEM4D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27516-1-PBS targets CD100 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26461

Product name: Recombinant human CD100 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 756-862 aa of BC054500

Sequence: YKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Calculated molecular weight 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberBC054500
Gene Symbol SEMA4D/CD100
Gene ID (NCBI) 10507
RRIDAB_2880896
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92854
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

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