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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCT5 Monoclonal antibody (67400-1-Ig)

    WB analysis using 67400-1-Ig (same clone as 67400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67400-1-Ig (CCT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CCT5 Monoclonal antibody (67400-1-Ig)

    WB analysis of HEK-293 using 67400-1-Ig (same clone as 67400-1-PBS)

    WB result of CCT5 antibody (67400-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells. This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CCT5 Monoclonal antibody (67400-1-Ig)

    IHC staining of human skin cancer using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67400-1-Ig (CCT5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCT5 Monoclonal antibody (67400-1-Ig)

    IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCT5 Monoclonal antibody (67400-1-Ig)

    IF Staining of HepG2 using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCT5 Monoclonal antibody (67400-1-Ig)

    IF Staining of U2OS using 67400-1-Ig (same clone as 67400-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCT5 antibody (67400-1-Ig, Clone: 1B12E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67400-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CCT5 Monoclonal antibody, PBS Only

CCT5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67400-1-PBS
Clone No.1B12E10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1B12E10, CCT epsilon, CCTE, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67400-1-PBS targets CCT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21421

Product name: Recombinant human CCT5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 243-541 aa of BC035499

Sequence: VEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE

相同性解析による交差性が予測される生物種
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Calculated molecular weight 541 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC035499
Gene Symbol CCT5
Gene ID (NCBI) 22948
RRIDAB_2882643
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP48643
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC). CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.