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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CCRL2 Monoclonal antibody (66611-1-Ig)

    WB analysis of HeLa using 66611-1-Ig (same clone as 66611-1-PBS)

    HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CCRL2 Monoclonal antibody (66611-1-Ig)

    WB analysis of A431 using 66611-1-Ig (same clone as 66611-1-PBS)

    A431 cells and K-562 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CCRL2 Monoclonal antibody (66611-1-Ig)

    IHC staining of human spleen using 66611-1-Ig (same clone as 66611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CCRL2 Monoclonal antibody (66611-1-Ig)

    IHC staining of human spleen using 66611-1-Ig (same clone as 66611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66611-1-PBS in a different storage buffer formulation.

CCRL2 Monoclonal antibody, PBS Only

CCRL2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66611-1-PBS
Clone No.2C1E11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

C C chemokine receptor like 2, CCR11, CCR6, CCRL2, Chemokine receptor CCR11, Chemokine receptor X, CKRX, CRAM, CRAM A, CRAM B, HCR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66611-1-PBS targets CCRL2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4045

Product name: Recombinant human CCRL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-344 aa of BC025717

Sequence: MWAPYNIAFFLSTFKEHFSLSDCKSSYNLDKSVHITKLIATTHCCINPLLYAFLDGTFSKYLCRCFHLRSNTPLQPRGQSAQGTSREEPDHSTEV

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor-like 2
Calculated molecular weight 344 aa, 40 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC025717
Gene Symbol CCRL2
Gene ID (NCBI) 9034
RRIDAB_2881971
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO00421
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chemokine CC motif receptor-like 2 (CCRL2) is a seven-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs) (PMID: 28743719). CCRL2 does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 has been found on almost all hemopoietic cells, including monocytes, macrophages, polymorphonuclear neutrophils (PMNs), T cells, dendritic cells, natural killer cells, and CD34+ progenitor cells, with PMNs expressing the highest levels (PMID: 15188357). CCRL2 is also expressed by barrier cells, such as lymphatic and blood endothelial cells and bronchial and intestinal epithelial cells (PMID: 29056935).

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