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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCR7 Recombinant monoclonal antibody (86180-5-RR)

    WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86180-5-RR (CCR7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CCR7 Recombinant monoclonal antibody (86180-5-RR)

    WB analysis using 86180-5-RR (same clone as 86180-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86180-5-RR (CCR7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CCR7 Recombinant monoclonal antibody (86180-5-RR)

    IF Staining of K-562 using 86180-5-RR (same clone as 86180-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCR7 antibody (86180-5-RR, Clone: 251886C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86180-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86180-5-RR

    Affinity and Kinetic Characterization of 86180-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86180-5-RR against Human CCR7 were performed. The affinity constant is 8.06 nM.

CCR7 Recombinant monoclonal antibody, PBS Only

CCR7 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86180-5-PBS
Clone No.251886C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BLR2, C C chemokine receptor type 7, C C CKR 7, C-C chemokine receptor type 7, CC CKR 7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86180-5-PBS targets CCR7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2354

Product name: recombinant human CCR7 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-59 aa of BC035343

Sequence: QDEVTDDYIGDNTTVDYTLFESLCSKKDVRNFKAW

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 7
Calculated molecular weight 378 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC035343
Gene Symbol CCR7
Gene ID (NCBI) 1236
RRIDAB_3744690
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32248
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C-C chemokine receptor type 7 (CCR7) , which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probablely acts as mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

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