CCR7 Polyclonal antibody
CCR7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25898-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells, C6 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 6 publications below |
| IF | See 4 publications below |
Product Information
25898-1-AP targets CCR7 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCR7 fusion protein Ag22941 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) receptor 7 |
| Calculated molecular weight | 378 aa, 43 kDa |
| Observed molecular weight | 40-43 kDa |
| GenBank accession number | BC035343 |
| Gene symbol | CCR7 |
| Gene ID (NCBI) | 1236 |
| RRID | AB_2880289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C-C chemokine receptor type 7 (CCR7), which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probably acts as a mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCR7 antibody 25898-1-AP | Download protocol |
| IHC protocol for CCR7 antibody 25898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold. | ||
Virulence Intranasal bovine β-defensin-5 enhances antituberculosis immunity in a mouse model by a novel protein-based respiratory mucosal vaccine. | ||
Front Cell Dev Biol Potential Mechanisms of the Impact of Hepatocyte Growth Factor Gene-Modified Tendon Stem Cells on Tendon Healing. | ||
Stem Cell Res Ther Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon. | ||
Front Mol Biosci CCR7 Has Potential to Be a Prognosis Marker for Cervical Squamous Cell Carcinoma and an Index for Tumor Microenvironment Change. | ||
Front Pharmacol MicroRNA-532-3p Suppresses Malignant Behaviors of Tongue Squamous Cell Carcinoma via Regulating CCR7. |
