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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCR3 Recombinant antibody (86038-1-RR)

    WB analysis using 86038-1-RR (same clone as 86038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86038-1-RR (CCR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86038-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CCR3 Recombinant antibody (86038-1-RR)

    IF Staining of K-562 using 86038-1-RR (same clone as 86038-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCR3 antibody (86038-1-RR, Clone: 250209D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86038-1-PBS in a different storage buffer formulation.

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CCR3 Recombinant antibody, PBS Only

CCR3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86038-1-PBS
Clone No.250209D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C C chemokine receptor type 3, C C CKR 3, C C CKR3, C-C chemokine receptor type 3, CC CKR 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86038-1-PBS targets CCR3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 3
Calculated molecular weight 355 aa, 41 kDa
Observed molecular weight53 kDa
GenBank accession numberBC110297
Gene Symbol CCR3
Gene ID (NCBI) 1232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51677
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCR3 (C-C Motif Chemokine Receptor 3), also known as CD193, is a G protein-coupled receptor (GPCR) primarily involved in immune cell trafficking and inflammation regulation and highly expressed in eosinophils and basophils, with detectable levels in Th2 cells, airway epithelial cells, and subsets of monocyte-derived dendritic cells (PMID: 21077277). Ligand-induced CCR3 internalization is required for eosinophil shape change and actin polymerization but not for calcium mobilization or desensitization (PMID: 12532103).