Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells, THP-1 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 24 publications below |
| IHC | See 14 publications below |
| IF | See 16 publications below |
| IP | See 1 publications below |
Product Information
16153-1-AP targets CCR2a-specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) receptor 2 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 36-42 kDa |
| GenBank accession number | NM_000647 |
| Gene Symbol | CCR2 |
| Gene ID (NCBI) | 729230 |
| RRID | AB_2262945 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| IF protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| IHC protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| WB protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Camouflaging attenuated Salmonella by cryo-shocked macrophages for tumor-targeted therapy | ||
Bone Res Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis. | ||
Nat Commun Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasis | ||
J Nanobiotechnology Engineered neutrophil membrane-camouflaged nanocomplexes for targeted siRNA delivery against myocardial ischemia reperfusion injury | ||
Theranostics LCN2 deficiency mitigates the neuroinflammatory damage following acute glaucoma |