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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCR2 Polyclonal antibody (30420-1-AP)

    WB analysis using 30420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30420-1-AP (CCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CCR2 Polyclonal antibody (30420-1-AP)

    IF Staining of THP-1 using 30420-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2 antibody (30420-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CCR2 Polyclonal antibody

CCR2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30420-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CC-CKR-2, C-C CKR-2, CC CKR 2, C-C chemokine receptor type 2, C C CKR 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, K-562 cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30420-1-AP targets CCR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCR2 fusion protein Ag33071 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) receptor 2
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight70 kDa
GenBank accession numberBC074751
Gene Symbol CCR2
Gene ID (NCBI) 729230
RRIDAB_3669721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR2 (C-C motif chemokine receptor-2), is a 42-kDa transmembrane protein highly expressed in bone marrow and lymph nodes (PMID: 16150057). It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1) (PMID: 8146186). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. (PMID: 19441905). This antibody recognizes a homodimer consisting of its isoform1 and isoform2 (70 kDa).

Protocols

Product Specific Protocols
WB protocol for CCR2 antibody 30420-1-APDownload protocol
IF protocol for CCR2 antibody 30420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols