Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 14067-1-AP (same clone as 14067-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 14067-1-AP (same clone as 14067-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)
IP result of anti-CCP1 (IP:14067-1-AP, 3ug; Detection:14067-1-AP 1:1000) with mouse testis tissue lysate 10000ug. This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14067-1-AP (CCP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 14067-1-AP (same clone as 14067-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 14067-1-AP (same clone as 14067-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 14067-1-AP (CCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14067-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CCP1 Polyclonal antibody, PBS Only

CCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14067-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14067-1-PBS targets CCP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5122 Product name: Recombinant human CCP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 837-1186 aa of BC060815 Sequence: VLCETLSGNSCPLVTITAMPESNYYEHICHFRNRPYVFLSARVHPGETNASWVMKGTLEYLMSNNPTAQSLRESYIFKIVPMLNPDGVINGNHRCSLSGEDLNRQWQSPSPDLHPTIYHAKGLLQYLAAVKRLPLVYCDYHGHSRKKNVFMYGCSIKETVWHTNDNATSCDVVEDTGYRTLPKILSHIAPAFCMSSCSFVVEKSKESTARVVVWREIGVQRSYTMESTLCGCDQGKYKGLQIGTRELEEMGAKFCVGLLRLKRLTSPLEYNLPSSLLDFENDLIESSCKVTSPTTYVLDEDEPRFLEEVDYSAESNDELDIELAENVGDYEPSAQEEVLSDSELSRTYLP 相同性解析による交差性が予測される生物種
Full Name	ATP/GTP binding protein 1
Calculated molecular weight	138 kDa
Observed molecular weight	138 kDa
GenBank accession number	BC060815
Gene Symbol	CCP1
Gene ID (NCBI)	23287
RRID	AB_2225559
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UPW5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 14067-1-AP (same clone as 14067-1-PBS)

WB analysis of A549 using 14067-1-AP (same clone as 14067-1-PBS)

WB analysis of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)

IP experiment of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of human testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of mouse testis using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of human heart using 14067-1-AP (same clone as 14067-1-PBS)

IHC staining of human heart using 14067-1-AP (same clone as 14067-1-PBS)

IF Staining of A549 using 14067-1-AP (same clone as 14067-1-PBS)

CCP1 Polyclonal antibody, PBS Only

CCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™