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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CCNY Monoclonal antibody

CCNY Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C9E3

Cat no : CL488-66865

Synonyms

C10orf9, CBCP1, CCNY, CFP1, Cyc Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin X, cyclin Y, FLJ95513

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using CL488-66865

    FC experiment of HEK-293 using CL488-66865

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CCNY (CL488-66865, Clone:2C9E3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66865-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66865 targets CCNY in FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCNY fusion protein Ag12691 相同性解析による交差性が予測される生物種
Full Name cyclin Y
Calculated molecular weight 33 kDa, 39 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC104773
Gene symbol CCNY
Gene ID (NCBI) 219771
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CCNY antibody CL488-66865Download protocol
Standard Protocols
Click here to view our Standard Protocols