Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
18042-1-AP targets CCNY in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12674 Product name: Recombinant human CCNY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC104773 Sequence: MEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin Y |
| Calculated molecular weight | 33 kDa, 39 kDa |
| Observed molecular weight | 33-39 kDa |
| GenBank accession number | BC104773 |
| Gene Symbol | CCNY |
| Gene ID (NCBI) | 219771 |
| RRID | AB_2074004 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8ND76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCNY, also named as C10orf9, CBCP1, CFP1, is a novel cyclin. CCNY acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCNY antibody 18042-1-AP | Download protocol |
| IHC protocol for CCNY antibody 18042-1-AP | Download protocol |
| IP protocol for CCNY antibody 18042-1-AP | Download protocol |
| WB protocol for CCNY antibody 18042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Prog Neurobiol Cyclin Y, a novel actin-binding protein, regulates spine plasticity through the cofilin-actin pathway. | ||
Prog Neurobiol Myristoylation-dependent palmitoylation of cyclin Y modulates long-term potentiation and spatial learning | ||
Pharmacol Res Ccny knockout mice display an enhanced susceptibility to kainic acid-induced epilepsy.
| ||
Biochem J Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16. | ||
Cells The Candidate IBD Risk Gene CCNY Is Dispensable for Intestinal Epithelial Homeostasis. | ||
