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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCNL2 Polyclonal antibody (11212-1-AP)

    WB analysis using 11212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11212-1-AP (CCNL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using CCNL2 Polyclonal antibody (11212-1-AP)

    WB analysis of A375 using 11212-1-AP

    WB result of CCNL2 antibody (11212-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCNL2 transfected A375 cells.

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  • KD/KO Validated

CCNL2 Polyclonal antibody

CCNL2 Polyclonal Antibody for WB, ELISA
Cat No. 11212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ANIA 6B, CCNL2, CCNM, cyclin L2, HCLA ISO, HLA ISO, PCEE, SB138

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, HeLa cells, U-251 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11212-1-AP targets CCNL2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCNL2 fusion protein Ag1710 相同性解析による交差性が予測される生物種
Full Name cyclin L2
Calculated molecular weight 58 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC016333
Gene Symbol CCNL2
Gene ID (NCBI) 81669
RRIDAB_2073623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCNL2 (Cyclin-L2) is also named as SB138 and Paneth cell-enhanced expression protein. Human cyclin L2 is a novel member of the cyclin family. Human cyclin L2 shares significant homology to cyclin L1, K, T1, T2, and C, which are involved in transcriptional regulation via phosphorylation of the C-terminal domain of RNA polymerase II (PMID: 14684736). Cyclin L2 co-localizes with splicing factors SC-35 and 9G8 within nuclear speckles and that it associates with hyperphosphorylated, but not hypophosphorylated, RNA polymerase II and CDK p110 PITSLRE kinase via its N-terminal cyclin domains (PMID: 14684736). Human cyclin L2 is expressed ubiquitously in normal human tissues and tumor cells.

Protocols

Product Specific Protocols
WB protocol for CCNL2 antibody 11212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols