Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
26270-1-AP targets CCM2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24233 Product name: Recombinant human CCM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-298 aa of BC004903 Sequence: ILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATEL 相同性解析による交差性が予測される生物種 |
| Full Name | cerebral cavernous malformation 2 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC004903 |
| Gene Symbol | CCM2 |
| Gene ID (NCBI) | 83605 |
| RRID | AB_2880454 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSQ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCM2 antibody 26270-1-AP | Download protocol |
| IHC protocol for CCM2 antibody 26270-1-AP | Download protocol |
| WB protocol for CCM2 antibody 26270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression.
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Biochem J CCM2 and PAK4 act downstream of atrial natriuretic peptide signaling to promote cell spreading.
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Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice
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Int Immunol Polymorphic tail of HLA-DQ leads to the heterogeneous IL-10 response of antigen-presenting cells. |