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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CCL5/RANTES Recombinant monoclonal antibody (88173-1-RR)

    WB analysis of human peripheral blood platelets using 88173-1-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 88173-1-RR (CCL5/RANTES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CCL5/RANTES Recombinant monoclonal antibody (88173-1-RR)

    WB analysis using 88173-1-RR

    PMA, LPS and Brefeldin A-treated THP-1 cells were subjected to SDS PAGE followed by western blot with 88173-1-RR (CCL5/RANTES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CCL5/RANTES Recombinant monoclonal antibody (88173-1-RR)

    WB analysis using 88173-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88173-1-RR (CCL5/RANTES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CCL5/RANTES Recombinant monoclonal antibody

CCL5/RANTES Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 88173-1-RR
Clone No.260534G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CCL5, C C motif chemokine 5, C-C motif chemokine 5, CCL 5, CCL-5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman peripheral blood platelets, NK-92 cells, PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88173-1-RR targets CCL5/RANTES in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2617

Product name: Recombinant human CCL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC008600

Sequence: MKVSAAALAVILIATALCAPASASPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 5
Calculated molecular weight 91 aa, 10 kDa
Observed molecular weight8 kDa
GenBank accession numberBC008600
Gene Symbol CCL5/RANTES
Gene ID (NCBI) 6352
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RANTES (CCL5) belongs to the CC chemokine family and induces leukocyte migration by binding to specific receptors in the G protein-coupled receptor family. RANTES (CCL5) has been shown in vitro to mediate eosinophil, lymphocyte, neutrophil, and monocyte chemotaxis, and it initiates several other proinflammatory events, such as integrin activation, lipid mediator biosynthesis, and degranulation.

Protocols

Product Specific Protocols
WB protocol for CCL5/RANTES antibody 88173-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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