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Validation Data Gallery

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  • Flow cytometry (FC) experiment of NR8383 cells using Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibo (98770-4-RR)

    FC experiment of NR8383 using 98770-4-RR

    1x10^6 untreated or LPS-treated NR8383 cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of NR8383 cells using Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibo (98770-4-RR)

    FC experiment of NR8383 using 98770-4-RR

    1x10^6 LPS treated NR8383 cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated NR8383 were surface stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibo (98770-4-RR)

    FC experiment of Transfected HEK-293T using 98770-4-RR

    1x10^6 HEK-293T or CCL4/MIP-1 beta transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibo (98770-4-RR)

    FC experiment of Transfected HEK-293T using 98770-4-RR

    1x10^6 HEK-293T or CCL4/MIP-1 beta transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibody

CCL4/MIP-1 beta Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98770-4-RR
Clone No.251973F1

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Synonyms

C-C motif chemokine 4, Ccl4, CCL-4, Macrophage inflammatory protein 1-beta, MIP-1 beta

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inLPS treated NR8383 cells, Transfected HEK-293T cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98770-4-RR targets CCL4/MIP-1 beta in FC (Intra) applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3200

Product name: recombinant rat CCL4/MIP-1 beta protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-92 aa of NM_053858.1

Sequence: APIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 4
Calculated molecular weight10 kDa
GenBank accession numberNM_053858.1
Gene Symbol Ccl4
Gene ID (NCBI) 116637
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP50230
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Protocols

Product Specific Protocols
FC protocol for CCL4/MIP-1 beta antibody 98770-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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