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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCL3 Polyclonal antibody (32190-1-AP)

    WB analysis using 32190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32190-1-AP (CCL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CCL3 Polyclonal antibody (32190-1-AP)

    IF Staining of THP-1 using 32190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using CCL3 antibody (32190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CCL3 Polyclonal antibody

CCL3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32190-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MIP-1-alpha, Mip1a, Macrophage inflammatory protein 1-alpha, L2G25B, Heparin-binding chemotaxis protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive IF/ICC detected inPMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32190-1-AP targets CCL3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 3
Calculated molecular weight10kd
Observed molecular weight11 kDa
GenBank accession numberNM_011337
Gene Symbol Ccl3
Gene ID (NCBI) 20302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP10855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCL3 antibody 32190-1-APDownload protocol
IF protocol for CCL3 antibody 32190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols