Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein, Transfected with Mouse Ccl19 Transfected HEK-293T cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85861-1-RR targets CCL19/MIP-3 beta in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3124 Product name: Recombinant Mouse CCL19/MIP-3 beta protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 26-108 aa of NM_011888.2 Sequence: GANDAEDCCLSVTQRPIPGNIVKAFRYLLNEDGCRVPAVVFTTLRGYQLCAPPDQPWVDRIIRRLKKSSAKNKGNSTRRSPVS 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) ligand 19 |
| Calculated molecular weight | 12 kDa |
| GenBank accession number | NM_011888.2 |
| Gene Symbol | Ccl19 |
| Gene ID (NCBI) | 24047 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O70460 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCL19/MIP-3 beta antibody 85861-1-RR | Download protocol |
| WB protocol for CCL19/MIP-3 beta antibody 85861-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |