Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
86565-1-PBS targets CCL12/MCP-5 in WB, Indirect ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3315 Product name: Recombinant Mouse CCL12/MCP-5 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-104 aa of NM_011331.2 Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) ligand 12 |
| Calculated molecular weight | 12KD |
| GenBank accession number | NM_011331.2 |
| Gene Symbol | Ccl12 |
| Gene ID (NCBI) | 20293 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q62401 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils.