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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of BxPC-3 using 16549-1-AP (same clone as 16549-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of SGC-7901 using 16549-1-AP (same clone as 16549-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using CCKBR-specific Polyclonal antibody (16549-1-AP)

    WB analysis of human stomach using 16549-1-AP (same clone as 16549-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16549-1-AP (CCKBR-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CCKBR-specific Polyclonal antibody (16549-1-AP)

    IHC staining of mouse brain using 16549-1-AP (same clone as 16549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CCKBR-specific Polyclonal antibody (16549-1-AP)

    IF Staining of BxPC-3 using 16549-1-AP (same clone as 16549-1-PBS)

    Immunofluorescent analysis of BxPC-3 cells using 16549-1-AP (CCKBR-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16549-1-PBS in a different storage buffer formulation.

CCKBR-specific Polyclonal antibody, PBS Only

CCKBR-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16549-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CCKBR, CCK B, CCK B receptor, CCK BR, CCK2 R

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16549-1-PBS targets CCKBR-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cholecystokinin B receptor
Calculated molecular weight 56 kDa
Observed molecular weight 64 kDa, 50 kDa
GenBank accession numberNM_176875
Gene Symbol CCKBR
Gene ID (NCBI) 887
RRIDAB_2070508
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32239
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The cholecystokinin B receptor (CCKBR) is a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

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