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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC91 Polyclonal antibody (31097-1-AP)

    WB analysis using 31097-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31097-1-AP (CCDC91 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC91 Polyclonal antibody (31097-1-AP)

    WB analysis of HeLa using 31097-1-AP

    WB result of CCDC91 antibody (31097-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC91 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCDC91 Polyclonal antibody (31097-1-AP)

    IF Staining of U2OS using 31097-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCDC91 antibody (31097-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

CCDC91 Polyclonal antibody

CCDC91 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31097-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

p56, HSD8, GGABP, GGA-binding partner, Coiled-coil domain-containing protein 91

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, rat testis tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31097-1-AP targets CCDC91 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34863

Product name: Recombinant human CCDC91 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC028682

Sequence: MPMWNKVSGVHLSPSSPEIVLDRDHSSSIGCLSSDAIISSPENTHAANSIVSQTIPKAQIQQSTHTHLDISLFPLGLTDEKSNGTIALVDDSEDPGANVSNIQLQQKISSLEIKLKVSEEEKQRIKQDVESLMEKHNVLEKGFLKEKEQEAISFQDRYKELQEKHKQELEDMRKAGHEALSIIVDEYKALLQSSVKQQVE

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 91
Observed molecular weight 50 kDa
GenBank accession numberBC028682
Gene Symbol CCDC91
Gene ID (NCBI) 55297
RRIDAB_3669854
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6B0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC91, also known as GGABP, p56, HSD8, is a critical accessory protein that promotes transport of carrier vesicles between the Golgi and lysosomes and lysosomal enzyme maturation (PMID: 17596511).

Protocols

Product Specific Protocols
IF protocol for CCDC91 antibody 31097-1-APDownload protocol
WB protocol for CCDC91 antibody 31097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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