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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC90B Polyclonal antibody (27126-1-AP)

    WB analysis using 27126-1-AP (same clone as 27126-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27126-1-AP (CCDC90B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CCDC90B Polyclonal antibody (27126-1-AP)

    IF Staining of A431 using 27126-1-AP (same clone as 27126-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CCDC90B antibody (27126-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC90B Polyclonal antibody (27126-1-AP)

    IF Staining of MCF-7 using 27126-1-AP (same clone as 27126-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCDC90B antibody (27126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27126-1-PBS in a different storage buffer formulation.

CCDC90B Polyclonal antibody, PBS Only

CCDC90B Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27126-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

MDS011, MDS025

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27126-1-PBS targets CCDC90B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25934

Product name: Recombinant human CCDC90B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 146-246 aa of BC014573

Sequence: IELDQVKQQLMHETSRIRADNKLDINLERSRVTDMFTDQEKQLMETTTEFTKKDTQTKSIISETSNKIDAEIASLKTLMESNKLETIRYLAASVFTCLAIA

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 90B
Observed molecular weight28 kDa
GenBank accession numberBC014573
Gene Symbol CCDC90B
Gene ID (NCBI) 60492
RRIDAB_3669584
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZT6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC90B (Coiled-coil domain-containing protein 90B) is predicted to have a single-pass transmembrane domain located at its C-terminal. As two isoforms belong to the same protein family, CCDC90A/MCUR1 and CCDC90B show similar expression levels among tissues with few exceptions.

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