Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32099-1-AP targets CCDC89 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36782 Product name: Recombinant human CCDC89 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC044814 Sequence: MRAPMLQKQQAPRMDTPPPEERLEKQNEKLNNQEEETEFKELDGLREALANLRGLSEEERSEKAMLRSRIEEQSQLICILKRRSDEALERCQILELLNAELEEKMMQEAEKLKAQGEYSRKLEERFMTLAANHELMLRFKDEYKSENIKLREENEKLRLENSSLFSQALKDEEAKVLQLTVRCEALTGELETLKERCAQD 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 89 |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC044814 |
| Gene Symbol | CCDC89 |
| Gene ID (NCBI) | 220388 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N998 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC89 (Coiled-coil domain-containing protein 89) is a type II transmembrane protein containing a typical coiled-coil domain, primarily localized to the Golgi apparatus and intracellular vesicle membranes. Its function has not yet been fully elucidated, but studies suggest that it acts as a "molecular scaffold" by mediating protein-protein interactions, playing roles in vesicular trafficking, cytoskeletal organization, and the assembly of multiprotein complexes, thereby maintaining cellular structural integrity and coordinating signal transduction. Currently, CCDC89 is emerging as a novel biomarker and potential therapeutic target bridging membrane trafficking, protein homeostasis, tumor biology, and reproductive medicine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC89 antibody 32099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |