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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC88C Polyclonal antibody

CCDC88C Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25769-1-AP

Synonyms

CCDC88C, DAPLE, hDaple, HkRP2, Hook related protein 2, KIAA1509, Protein Daple

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 25769-1-AP

    WB analysis of HEK-293 using 25769-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25769-1-AP (CCDC88C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25769-1-AP

    IHC staining of human testis using 25769-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 25769-1-AP

    IHC staining of human testis using 25769-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25769-1-AP (CCDC88C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 25769-1-AP

    IF Staining of HEK-293 using 25769-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 25769-1-AP (CCDC88C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25769-1-AP is CCDC88C Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC88C fusion protein Ag22711 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 88C
Calculated molecular weight 2028 aa, 228 kDa
Observed molecular weight 250 kDa, 100 kDa
GenBank accession numberBC028565
Gene symbol CCDC88C
Gene ID (NCBI) 440193
RRIDAB_2880233
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC88C antibody 25769-1-APDownload protocol
IHC protocol for CCDC88C antibody 25769-1-APDownload protocol
IF protocol for CCDC88C antibody 25769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Authors - Katie S Kindt
View Article
mouse

Sci Adv

RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells

Authors - Anil Akturk
View Article