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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using CCDC85B Polyclonal antibody (18282-1-AP)

    WB analysis of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CCDC85B Polyclonal antibody (18282-1-AP)

    WB analysis of mouse lung using 18282-1-AP (same clone as 18282-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using CCDC85B Polyclonal antibody (18282-1-AP)

    IP experiment of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

    IP result of anti-CCDC85B (IP:18282-1-AP, 4ug; Detection:18282-1-AP 1:300) with mouse heart tissue lysate 3600ug. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CCDC85B Polyclonal antibody (18282-1-AP)

    IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CCDC85B Polyclonal antibody (18282-1-AP)

    IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC85B Polyclonal antibody (18282-1-AP)

    IF Staining of HeLa using 18282-1-AP (same clone as 18282-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18282-1-AP (CCDC85B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

CCDC85B Polyclonal antibody, PBS Only

CCDC85B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18282-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18282-1-PBS targets CCDC85B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13129

Product name: Recombinant human CCDC85B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC008796

Sequence: MEAEAGGLEELTDEEMAALGKEELVRRLRREEAARLAALVQRGRLMQEVNRQLQGHLGEIRELKQLNRRLQAENRELRDLCCFLDSERQRGRRAARQWQLFGTQASRAVREDLGGCWQKLAELEGRQEELLRENLALKELCLALGEEWGPRGGPSGAGGSGAGPAPELALPPCGPRDLGDGSSSTGSVGSPDQLPLACSPDD

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 85B
Calculated molecular weight 22 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC008796
Gene Symbol CCDC85B
Gene ID (NCBI) 11007
RRIDAB_2878527
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15834
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.

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