Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A549 using 20393-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20393-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20393-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20393-1-AP (CCDC72 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 20393-1-AP
IP result of anti-CCDC72 (IP:20393-1-AP, 4ug; Detection:20393-1-AP 1:500) with A549 cells lysate 2400 ug.
IHC staining of human skin using 20393-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 20393-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 20393-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 20393-1-AP (CCDC72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CCDC72 Polyclonal antibody

CCDC72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20393-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Translation machinery-associated protein 7, TMA7, HSPC330, HSPC016, Coiled-coil domain-containing protein 72

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, MCF-7 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

20393-1-AP targets CCDC72 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13792 Product name: Recombinant human CCDC72 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-64 aa of BC001102 Sequence: MSGREGGKKKPLKQPKKQAKEMDEEDKAFKQKQKEEQKKLEELKAKAAGKGPLATGGIKKSGKK 相同性解析による交差性が予測される生物種
Full Name	coiled-coil domain containing 72
Calculated molecular weight	64 kDa, 7 kDa
Observed molecular weight	7 kDa
GenBank accession number	BC001102
Gene Symbol	CCDC72
Gene ID (NCBI)	51372
RRID	AB_10667404
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2S6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCDC72, also named as TMA7, is a novel modulator for hair follicle development. CCDC72 has the promoter activity and regulation of gene expression.

Protocols

Product Specific Protocols
WB protocol for CCDC72 antibody 20393-1-AP	Download protocol
IHC protocol for CCDC72 antibody 20393-1-AP	Download protocol
IF protocol for CCDC72 antibody 20393-1-AP	Download protocol
IP protocol for CCDC72 antibody 20393-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Int J Biol Sci IGF2BP3 Regulates TMA7-mediated Autophagy and Cisplatin Resistance in Laryngeal Cancer via m6A RNA Methylation Authors - Like Yang View Article
mouse	WB	bioRxiv Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletion Authors - Shiyu Luo View Article
mouse	WB	Dis Model Mech HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells Authors - Shiyu Luo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 20393-1-AP

WB analysis of MCF-7 using 20393-1-AP

WB analysis of HEK-293 using 20393-1-AP

IP experiment of A549 using 20393-1-AP

IHC staining of human skin using 20393-1-AP