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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC71 Polyclonal antibody

CCDC71 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28152-1-AP

Synonyms

CCDC71, FLJ12800, FLJ13629

Validation Data Gallery

Applications:
  • WB analysis using 28152-1-AP

    WB analysis using 28152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28152-1-AP (CCDC71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28152-1-AP

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28152-1-AP

    IHC staining of human liver cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28152-1-AP

    IHC staining of human liver cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28152-1-AP targets CCDC71 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC71 fusion protein Ag27446 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 71
Calculated molecular weight 467 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC035516
Gene symbol CCDC71
Gene ID (NCBI) 64925
RRIDAB_2881075
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC71 antibody 28152-1-APDownload protocol
IHC protocol for CCDC71 antibody 28152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols