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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC71 Polyclonal antibody (28152-1-AP)

    WB analysis using 28152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28152-1-AP (CCDC71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC71 Polyclonal antibody (28152-1-AP)

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC71 Polyclonal antibody (28152-1-AP)

    IHC staining of human breast cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC71 Polyclonal antibody (28152-1-AP)

    IHC staining of human liver cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCDC71 Polyclonal antibody (28152-1-AP)

    IHC staining of human liver cancer using 28152-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28152-1-AP (CCDC71 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CCDC71 Polyclonal antibody

CCDC71 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Synonyms

CCDC71, FLJ12800, FLJ13629

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28152-1-AP targets CCDC71 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC71 fusion protein Ag27446 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 71
Calculated molecular weight 467 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC035516
Gene Symbol CCDC71
Gene ID (NCBI) 64925
RRIDAB_2881075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC71 antibody 28152-1-APDownload protocol
IHC protocol for CCDC71 antibody 28152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols