Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, U2OS cells, MDA-MB-231 cells, RT-4 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31457-1-AP targets CCDC69 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CCDC69 fusion protein Ag34858 相同性解析による交差性が予測される生物種 |
Full Name | coiled-coil domain containing 69 |
Observed molecular weight | 43-45 kDa |
GenBank accession number | BC016647 |
Gene Symbol | CCDC69 |
Gene ID (NCBI) | 26112 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | A6NI79 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC69 (Coiled-coil domain-containing protein 69), may act as a scaffold to regulate the recruitment and assembly of spindle midzone components, which is required for the localization of AURKB and PLK1 to the spindle midzone (PMID: 20962590). It is highly expressed in duodenum, esophagus, pancreas, prostate, salivary gland, thymus and urinary bladder. The molecular weight of CCDC69 is 34 kDa, and it has been reported that the protein has a molecular weight of 43kDa (PMID: 20962590).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CCDC69 antibody 31457-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |