Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26333-1-AP targets CCDC66 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23421 Product name: Recombinant human CCDC66 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC132827 Sequence: MTFSSTKDLCKQCIDKDCLHIQKEISPATPNMQKTRNTVNTSLVGKQKPHKKHITAENMKSSLVCLTQDQLQQILMTVNQGNRSLSLTENGKEAKSQYSLYLNSISNQPKDENIMGLFKKTEMVSSVPAENKSVLNEHQETSKQCEQKIAIENEWKPADIFSTLGERECDRSSLEAKKAQWRKELDEQVALKKKEKEVSEKWNDPWKKSESDKIIWEKHQILDQSRETVLLEHPFSAVKQELQRKWIEELNKQIEDDRQRKIEEKIIYSKGEEHDRWAMHFDSLKSYPGSQSQLFSRSTHKQPEYFCVSPDTQELADVSSVCTPTTGSQVEPSEEEHIAKPIKDVVMANSKKTNFLRSMTALLD 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 66 |
| Observed molecular weight | 105-125 kDa |
| GenBank accession number | BC132827 |
| Gene Symbol | CCDC66 |
| Gene ID (NCBI) | 285331 |
| RRID | AB_3742183 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A2RUB6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC66 is a MAP that localizes to the centriolar satellites, the centrosome and the primary cilium and is linked in ciliopathies affecting retina and brain. It interacts with the ciliopathy proteins CEP104 and CSPP1 and controls axonemal elongation (PMID: 36606424). CCDC66 is required for the basal body recruitment of transition zone proteins and intraflagellar transport B (IFT-B) machinery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCDC66 antibody 26333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |