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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC64B Polyclonal antibody (22770-1-AP)

    WB analysis using 22770-1-AP

    MCF-7 lysates were subjected to SDS PAGE followed by western blot with 22770-1-AP (CCDC64B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC64B Polyclonal antibody (22770-1-AP)

    IHC staining of human breast cancer using 22770-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22770-1-AP (CCDC64B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CCDC64B Polyclonal antibody (22770-1-AP)

    IHC staining of human breast cancer using 22770-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22770-1-AP (CCDC64B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CCDC64B Polyclonal antibody

CCDC64B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22770-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Bicaudal D related protein 2, BICD related protein 2, BICDR 2, BICDR2, CCDC64B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22770-1-AP targets CCDC64B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC64B fusion protein Ag18720 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 64B
Calculated molecular weight 508 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC128602
Gene Symbol CCDC64B
Gene ID (NCBI) 146439
RRIDAB_2918078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1A5D9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC64B, also named as BICDL2 and BICDR2, belongs to BICD family. It was officially named by Schlager et al (2010) in a sequence homology search for new mammalian BICD-like proteins. At present, there is still little study on this protein, which may involve the vesicle transport along microtubule, and Golgi to secretory granule transport. BICDR2 has 2 isoforms with the molecular mass of 57 and 34 kDa (PMID:20360680, 20485297).

Protocols

Product Specific Protocols
WB protocol for CCDC64B antibody 22770-1-APDownload protocol
IHC protocol for CCDC64B antibody 22770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols