Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33600-1-AP targets CCDC63 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39252 Product name: Recombinant human CCDC63 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of NM_152591.2 Sequence: MSVLKKNRRKDSDTPQEPSEKAKEQQAEAELRKLRQQFRKMVESRKSFKFRNQQKIASQYKEIKTLKTEQDEITLLLSLMKSSRNMNRSEKNYMELRLLLQTKEDYEALIKSLKVLLAELDEKILQMEKKIANQKQIFAKMQEANNPRKLQKQIHILETRLNLVTVHFDKMLTTNAKLRKEIEDLRFEKAAYDNVYQQLQ 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 63 |
| Calculated molecular weight | 66kDa，563aa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | NM_152591.2 |
| Gene Symbol | CCDC63 |
| Gene ID (NCBI) | 160762 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8NA47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC63 (Coiled-Coil Domain Containing 63) is a highly conserved centrosomal satellite protein whose main functions are closely related to cell mitosis and ciliogenesis. This protein directly interacts with PCM1, a key organizer of centrosomal satellites, through its coiled-coil domain and is recruited to the pericentriolar material. As an important component of centrosomal satellites, CCDC63 is involved in regulating the normal assembly of the centrosome, microtubule anchoring, and proper orientation of the spindle, thereby ensuring the fidelity of chromosome segregation and genomic stability. Therefore, CCDC63 is one of the core molecules that maintain the accuracy of cell division and the function of organelles.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for CCDC63 antibody 33600-1-AP | Download protocol |
| WB protocol for CCDC63 antibody 33600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |