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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC59 Polyclonal antibody

CCDC59 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26387-1-AP

Synonyms

BR22, CCDC59, HSPC128, TAP26, TTF 1 associated protein 26, TTF 1 associated protein BR2

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 26387-1-AP

    WB analysis of MCF-7 using 26387-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26387-1-AP (CCDC59 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 26387-1-AP

    IP experiment of HepG2 using 26387-1-AP

    IP result of anti-CCDC59 (IP:26387-1-AP, 4ug; Detection:26387-1-AP 1:1000) with HepG2 cells lysate 4000ug.

  • IHC staining of human lung cancer using 26387-1-AP

    IHC staining of human lung cancer using 26387-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26387-1-AP (CCDC59 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26387-1-AP

    IHC staining of human lung cancer using 26387-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26387-1-AP (CCDC59 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26387-1-AP is CCDC59 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC59 fusion protein Ag23852 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 59
Observed molecular weight 34-38 kDa
GenBank accession numberBC020647
Gene symbol CCDC59
Gene ID (NCBI) 29080
RRIDAB_2880499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC59 antibody 26387-1-APDownload protocol
IHC protocol for CCDC59 antibody 26387-1-APDownload protocol
IP protocol for CCDC59 antibody 26387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols