Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
26387-1-AP targets CCDC59 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23852 Product name: Recombinant human CCDC59 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-241 aa of BC020647 Sequence: MAPVRRSAKWRPGGIEARGEGVSTVGYRNKNVRQKTWRPNHPQAFVGSVREGQGFAFRRKLKIQQSYKKLLRKEKKAQTSLESQFTDRYPDNLKHLYLAEEERHRKQARKVDHPLSEQVHQPLLEEQCSIDEPLFEDQCSFDQPQPEEQCIKTVNSFTIPKKNKKKTSNQKAQEEYEQIQAKRAAKKQEFERRKQEREEAQRQYKKKKMEVFKILNKKTKKGQPNLNVQMEYLLQKIQEKC 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 59 |
| Observed molecular weight | 34-38 kDa |
| GenBank accession number | BC020647 |
| Gene Symbol | CCDC59 |
| Gene ID (NCBI) | 29080 |
| RRID | AB_2880499 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC59 (Coiled-Coil Domain Containing 59), also known as TAP26 (Thyroid transcription factor 1-associated protein 26), is a core, essential protein heavily involved in cellular RNA-handling and protein metabolism. It forms a component of the transcription complexes that direct the production of pulmonary surfactant-associated proteins B (SFTPB) and C (SFTPC), which are vital for maintaining surface tension in lung alveoli. CCDC59 is primarily localized within the nucleoplasm and nucleolus, while minor distributions can be found in the cytoplasm and plasma membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCDC59 antibody 26387-1-AP | Download protocol |
| IP protocol for CCDC59 antibody 26387-1-AP | Download protocol |
| WB protocol for CCDC59 antibody 26387-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |