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Host
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC55 Polyclonal antibody

CCDC55 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21360-1-AP

Synonyms

CCDC55, HSPC095

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 21360-1-AP

    WB analysis of COLO 320 using 21360-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21360-1-AP (CCDC55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21360-1-AP

    IHC staining of human brain using 21360-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 21360-1-AP

    IHC staining of human brain using 21360-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21360-1-AP (CCDC55 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21360-1-AP is CCDC55 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC55 fusion protein Ag15811 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 55
Calculated molecular weight 558 aa, 66 kDa
Observed molecular weight 66 kDa 70 kDa
GenBank accession numberBC040118
Gene symbol CCDC55
Gene ID (NCBI) 84081
RRIDAB_2878847
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC55, also named as NSRP1 and NSRP70, is a conserved coiled-coil domain containing protein. It is a novel regulator of cell migration. This antibody detects 66 kDa (native form) and 70 kDa (phospho form).

Protocols

Product Specific Protocols
WB protocol for CCDC55 antibody 21360-1-APDownload protocol
IHC protocol for CCDC55 antibody 21360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Int J Mol Sci

Comprehensive Atlas of Alternative Splicing Reveals NSRP1 Promoting Adipogenesis through CCDC18

Authors - Lei Liu
  • KD Validated
View Article