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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC33 Polyclonal antibody (25822-1-AP)

    WB analysis using 25822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25822-1-AP (CCDC33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CCDC33 Polyclonal antibody (25822-1-AP)

    IP experiment of mouse testis using 25822-1-AP

    IP result of anti-CCDC33 (IP:25822-1-AP, 4ug; Detection:25822-1-AP 1:600) with mouse testis tissue lysate 1800 ug.

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CCDC33 Polyclonal antibody

CCDC33 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25822-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Cancer/testis antigen 61, CT61

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25822-1-AP targets CCDC33 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC33 fusion protein Ag22893 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 33
Calculated molecular weight 958 aa, 107 kDa
Observed molecular weight85 kDa
GenBank accession numberBC025689
Gene Symbol CCDC33
Gene ID (NCBI) 80125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5R6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human CCDC33 gene was reported to encode a cancer/testis (CT) antigen, which is located on 15q24.1, spans a region of 99.8 kb, and consists of 20 exons (PMID: 28639886). CCDC33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 5 different transcripts (37kda, 41kda, 85kda, 107kda, 114kda). Human CCDC33 was reported to be a cancer/testis (CT) protein. Abundant expression of Ccdc33 is evident during mouse spermatogenesis (PMID: 33942254).

Protocols

Product Specific Protocols
WB protocol for CCDC33 antibody 25822-1-APDownload protocol
IP protocol for CCDC33 antibody 25822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols