Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, Jurkat cells, A375 cells, mouse thymus tissue, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86883-1-RR targets CCDC28A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6529 Product name: Recombinant human CCDC28A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC000758 Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 28A |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC000758 |
| Gene Symbol | CCDC28A |
| Gene ID (NCBI) | 25901 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWP9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCDC28A antibody 86883-1-RR | Download protocol |
| IHC protocol for CCDC28A antibody 86883-1-RR | Download protocol |
| WB protocol for CCDC28A antibody 86883-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |