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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of HeLa using 14807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CCDC28A antibody 14807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CCDC28A Polyclonal antibody

CCDC28A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14807-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C6orf80, CC28A, CCRL1AP, Coiled-coil domain-containing protein 28A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14807-1-AP targets CCDC28A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6529

Product name: Recombinant human CCDC28A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC000758

Sequence: MPKKNAIPVSKSTGFSNPASQSTSQRPKLKRVMKEKTKPQGGEGKGAQSTPIQHSFLTDVSDVQEMERGLLSLLNDFHSGKLQAFGNECSIEQMEHVRGMQEKLARLNLELYGELEELPEDKRKTASDSNLDRLLSDLEELNSSIQKLHLADAQDVPNTSAS

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 28A
Calculated molecular weight 30 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000758
Gene Symbol CCDC28A
Gene ID (NCBI) 25901
RRIDAB_2072069
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coiled-coil domain-containing protein 28A (CCDC28A) is a translocation partner of nucleoporin 98 (NUP98) in acute leukemias, resulting in the creation of fusion genes and the expression of chimeric proteins. These fusion proteins, such as NUP98-CCDC28A, have been shown to promote myeloproliferative neoplasms (PMID: 22058212). Additionally, CCDC28A is essential for head-tail coupling stability, thereby ensuring sperm motility (PMID: 39500989).

Protocols

Product Specific Protocols
IF protocol for CCDC28A antibody 14807-1-APDownload protocol
WB protocol for CCDC28A antibody 14807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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