Validation Data Gallery

Filter: AllWBIP

WB analysis of human brain using 16636-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 16636-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16636-1-AP
WB result of CCDC22 antibody (16636-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.
WB analysis of rat colon using 16636-1-AP
rat colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16636-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 16636-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 16636-1-AP
IP result of anti-CCDC22 (IP:16636-1-AP, 4ug; Detection:16636-1-AP 1:600) with mouse lung tissue lysate 3600ug.

Featured Product
KD/KO Validated

CCDC22 Polyclonal antibody

CCDC22 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16636-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Synonyms

JM1, CXorf37, Coiled-coil domain-containing protein 22, Coiled coil domain containing protein 22, coiled coil domain containing 22

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (20)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, HepG2 cells, mouse colon tissue, mouse lung tissue, rat colon tissue, HeLa cells, Jurkat cells
Positive IP detected in	mouse lung tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 17 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

16636-1-AP targets CCDC22 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9980 Product name: Recombinant human CCDC22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 283-628 aa of BC000972 Sequence: AKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQWEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYLAALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA 相同性解析による交差性が予測される生物種
Full Name	coiled-coil domain containing 22
Calculated molecular weight	71 kDa
Observed molecular weight	71 kDa
GenBank accession number	BC000972
Gene Symbol	CCDC22
Gene ID (NCBI)	28952
RRID	AB_2072065
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60826
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IP protocol for CCDC22 antibody 16636-1-AP	Download protocol
WB protocol for CCDC22 antibody 16636-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells. Authors - Zharko Daniloski View Article
human	WB	Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. Authors - Kerrie E McNally View Article
human	WB	Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Authors - Yunkai Zhu View Article
mouse	WB	Nat Commun CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Authors - Paulina Bartuzi View Article
human	WB, IP	J Cell Biol Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling. Authors - Haiying Li View Article
human	WB	Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. Authors - Liu Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 16636-1-AP

WB analysis using 16636-1-AP

WB analysis of HepG2 using 16636-1-AP

WB analysis of rat colon using 16636-1-AP

WB analysis of mouse lung using 16636-1-AP

WB analysis of mouse colon using 16636-1-AP

IP experiment of mouse lung using 16636-1-AP

CCDC22 Polyclonal antibody

CCDC22 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.