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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis using 21390-1-AP (same clone as 21390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of COLO 320 using 21390-1-AP (same clone as 21390-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of HepG2 using 21390-1-AP (same clone as 21390-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of L02 using 21390-1-AP (same clone as 21390-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    WB analysis of human brain using 21390-1-AP (same clone as 21390-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 21390-1-AP (CCDC153 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    IHC staining of human prostate hyperplasia using 21390-1-AP (same clone as 21390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using CCDC153 Polyclonal antibody (21390-1-AP)

    IHC staining of human prostate hyperplasia using 21390-1-AP (same clone as 21390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21390-1-AP (CCDC153 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCDC153 Polyclonal antibody (21390-1-AP)

    IF Staining of HepG2 using 21390-1-AP (same clone as 21390-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CCDC153 antibody 21390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC153 Polyclonal antibody (21390-1-AP)

    IF Staining of HeLa using 21390-1-AP (same clone as 21390-1-PBS)

    Immunofluorescent analysis of Hela cells, using CCDC153 antibody 21390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 21390-1-PBS in a different storage buffer formulation.

CCDC153 Polyclonal antibody, PBS Only

CCDC153 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21390-1-PBS targets CCDC153 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16044

Product name: Recombinant human CCDC153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-124 aa of BC101443

Sequence: MSRQCHALQEDMQTHSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYEEILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLTPPGSLRPPAPSL

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 153
Calculated molecular weight 210 aa, 24 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC101443
Gene Symbol CCDC153
Gene ID (NCBI) 283152
RRIDAB_10858621
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ494R4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC153's properties have yet to be elucidated. However, research suggests that it may be a neuronal subtype marker (PMID: 28166221). It has a molecular weight of 24 KDa and is often observed on a Western blot as a dimer.

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