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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC136 Polyclonal antibody (21351-1-AP)

    WB analysis using 21351-1-AP (same clone as 21351-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21351-1-AP (CCDC136 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CCDC136 Polyclonal antibody (21351-1-AP)

    IP experiment of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    IP result of anti-CCDC136 (IP:21351-1-AP, 4ug; Detection:21351-1-AP 1:800) with mouse testis tissue lysate 1800 ug. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC136 Polyclonal antibody (21351-1-AP)

    IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CCDC136 Polyclonal antibody (21351-1-AP)

    IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CCDC136 Polyclonal antibody (21351-1-AP)

    IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CCDC136 Polyclonal antibody (21351-1-AP)

    IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

CCDC136 Polyclonal antibody, PBS Only

CCDC136 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21351-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21351-1-PBS targets CCDC136 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15737

Product name: Recombinant human CCDC136 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 461-810 aa of BC109220

Sequence: AELQHLRDTVASFKESNEKDTETHAQLQEMKQLYQASKDELERQKHMYDQLEQDLLLCQLELKELKASHPIPEDKGKCANKCDTLLSRLTELQEKYKASQKEMGQLQMEQCELLEDQRRMQEEQGQLQEELHRLTLPLPKSGLLLKSQELLTKLEDLCELQLLYQGMQEEQKKLIQNQDCVLKEQLEIHEELRRFKESHFQEVLENPDDSKLAKSSKCNRNKQSKLLMEQMQALQVMYDAGQAKQELLQQEQGRLLEERKRLQADLQLCLEEMQLLQVQSPSIKMSLESYGKSYGSMVPSNENCRKTYDTTVDDNESYYKSYTSTQTSSKSFLKSYDSSTSASEAYGKSY

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 136
Calculated molecular weight 1154 aa, 134 kDa
Observed molecular weight110 kDa, 134 kDa
GenBank accession numberBC109220
Gene Symbol CCDC136
Gene ID (NCBI) 64753
RRIDAB_3742029
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JN2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC136 (also known as NAG6) is a coiled-coil domain containing protein described as a potential tumor suppressor in gastric cancer. CCDC136 protein was most abundantly located in the acrosome of round spermatids and elongating spermatids within seminiferous tubules of the adult mouse testis (PMID: 27076447).

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