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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24026-1-AP

Synonyms

CCDC105

Validation Data Gallery

Applications:
  • WB analysis of DU 145 using 24026-1-AP

    WB analysis of DU 145 using 24026-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 24026-1-AP

    WB analysis of SH-SY5Y using 24026-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24026-1-AP

    WB analysis of HeLa using 24026-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 24026-1-AP

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24026-1-AP

    IF Staining of HeLa using 24026-1-AP

    Immunofluorescent analysis of HeLa cells using 24026-1-AP (CCDC105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24026-1-AP

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

24026-1-AP targets CCDC105 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC105 fusion protein Ag21208 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 105
Calculated molecular weight 499 aa, 57 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC035678
Gene symbol CCDC105
Gene ID (NCBI) 126402
RRIDAB_2879406
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC105 antibody 24026-1-APDownload protocol
IHC protocol for CCDC105 antibody 24026-1-APDownload protocol
IF protocol for CCDC105 antibody 24026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols