Validation Data Gallery
Tested Applications
| Positive IP detected in | SH-SY5Y cells, MOLT-4 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31675-1-AP targets CCDC102B in IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36379 Product name: Recombinant human CCDC102B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC126448 Sequence: MKPNLDGVDLFNNGGSGNGETKTGLRLKAINLPLENEVTEISALQVHLDEFQKILWKEREMRTALEKEIERLESALSLWKWKYEELKESKPKNVKEFDILLGQHNDEMQELSGNIKEESKSQNSKDRVICELRAELERLQAENTSEWDKREILEREKQGLERENRRLKIQVKEMEELLDKKNRLSANSQSPDFKMSQIDLQEKNQELLNLQHAYYKLNRQYQANIAELTHANNRVDQNEA 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 102B |
| Observed molecular weight | 60~68 kDa |
| GenBank accession number | BC126448 |
| Gene Symbol | CCDC102B |
| Gene ID (NCBI) | 79839 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q68D86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCDC102B antibody 31675-1-AP | Download protocol |
| IP protocol for CCDC102B antibody 31675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |