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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCBL2 Polyclonal antibody (55476-1-AP)

    WB analysis using 55476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55476-1-AP (CCBL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CCBL2 Polyclonal antibody (55476-1-AP)

    IF Staining of K-562 using 55476-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCBL2 antibody (55476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CCBL2 Polyclonal antibody

CCBL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55476-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RBMXL1, KATIII, KAT3, GTL, Glutamine Transaminase L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55476-1-AP targets CCBL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cysteine conjugate-beta lyase 2
Calculated molecular weight 51 kDa
Observed molecular weight48 kDa，51- 55 kda
GenBank accession numberNM_019610
Gene Symbol CCBL2
Gene ID (NCBI) 56267
RRIDAB_3670254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YP21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cysteine conjugate beta-lyase 2 (CCBL2), also known as kynurenine aminotransferase 3 (KAT3) or glutamine transaminase L (GTL), can effectively catalyze the transamination of glutamine, methionine, and kynurenine (KYN) to kynurenic acid (KYNA) as well as the pathway of drug metabolism by cytochrome P450. CCBL2 has been found in mouse, rat, and human whose mRNA is widely expressed in several organs such as the liver, kidney, heart, and neuroendocrine tissues (PMID: 35771747).

Protocols

Product Specific Protocols
WB protocol for CCBL2 antibody 55476-1-APDownload protocol
IF protocol for CCBL2 antibody 55476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols